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Volume 162 - Anno 2011 - Numero 6

Low dose of tocotrienols protects osteoblasts against oxidative stress


di A.M. Nizar, A.S. Nazrun, M. Norazlina, M. Norliza, S. Ima Nirwana

Aims. Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. This in vitro study was conducted to determine the protective effects of α-tocopherol and γ-tocotrienol on osteoblasts, the bone forming cells, against oxidative stress. Materials and Methods. Toxicity tests were carried out on hydrogen peroxide (H2O2), α-tocopherol and γ-tocotrienol and their inhibitory concentration 50 (IC50) on osteoblasts were determined if any. Osteoblast cultures were then pretreated with different concentrations of α-tocopherol or γ-tocotrienol for 24 hours before incubated with the IC50 of H2O2 for 2 hours. Cell viability was determined by using MTS assay to compare the protective effects of both vitamin E on osteoblast exposed to H2O2. Results. The IC50 after 2 hours and 24 hours incubation time for H2O2 were 490 ÁM and 280 ÁM respectively. γ-Tocotrienol was found to be toxic to osteoblasts with the IC 50 of 290 ÁM after 24 hours incubation time while α-tocopherol was not toxic to osteoblasts at any doses. However, γ-tocotrienol was able to protect osteoblasts from H2O2 toxicity at low concentration (1 ÁM), whereras α-tocopherol was not able to offer protection against H2O2 toxicity. Conclusions. γ-tocotrienol was found to be toxic to osteoblasts at high concentrations but at much lower concentration, it has better antioxidant activity than α-tocopherol to protect osteoblasts from oxidative stress. Clin Ter 2011; 162(6):533-538

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